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[KARYA1]

The Potential of Chili Seed (Capsicum Annuum L.) As the Modality of Vulvovaginal Candidiasis Theraphy : A Study using Protein Extraction Method

Diah Nurul Islami Muchsin1, A. Tazkiyah Batari Uleng1, Widarsi1, Mirsha Hijriana1, Nurul Oktariani Azis1

 

Abstract

Vulvovaginal Candidiasis (VVC) is one of gynecology disease that happen to woman at reproductive age. According to research, Candida albicans was found around 80-90% of VVC cases, caused disturbance of normal flora in vagina. The research in Ilam-Iran showed that 150 isolat of Candida albicans had become resistant to its medicine. According to the case, another research showed that herbal material from plant can be an alternative medicine towards Candida albicans, one of them is chili (Capsisum annuum L.) that contains a group of protein compounds known as CaThi that belong to Plant Antimicrobial Peptides (AMPs) group which act as the defense mechanism of plant against microorganism. So, the aim of this research is to find the potential of chili seed compound using protein extraction method as the modality to treat VVC. This research used laboratorium experimental method with in vitro effectivity test by using Sabouraud Dextrose Agar (SDA) in 100%, 50%, 25%, 12,5%, and 6,25% concentration. The compound of chili seed is obtained from protein isolation and fractionation method. Isolation method done by smoothing the chili seed and reviled by citric buffer at 5,4 pH. The Solution from this process is continued by fractionation method at 20% and 40% saturation. The sedimentation that got after it left a night will be tested by measure the diameter of inhibition zone. Citric buffer used as negative control and ketoconazole as positive control.

The result of diameter of inhibition zone at 20% saturation with 100%, 50%, 25%, 12,5%, and 6,25% concentration showed 13,65 mm, 9,35 mm, 8,6 mm, 8,55 mm, and 8,5 mm. Another result at 40% saturation showed 10,35 mm, 9,55 mm, 8,35mm, 7,55 mm and 7,9 mm. The control positive diameter is 24 mm and negative control 0 mm. So, this research shows that there is potency of chili seed extract against Candida albicans to treat VVC.

Keywords: Candida albicans, Chili (Capsicum annuum L.), Vulvovaginalis Candidiasis.

 

Author information

Affiliations

Faculty of Medicine, Hasanuddin University

Corresponding author

Correspond to: Diah Nurul Islami Muchsin (diahnurulislami@ymail.com)